50. Mueller, J.; Krishnan, J.; Wei, Q.; Hefner, Y.; Monk, J.M.; Verkler, J.; Tibocha-Bonilla, J.D.; Ayala, A.; Palsson, B.O.; Feist, A.M.*; Niu, W.* “Multi-strain analysis of Pseudomonas putida reveals the metabolic and genetic diversity of the species” mSystems 2026.
49. Shaik, S.P.; Li, A.; Guo, J.*; Niu, W.* “Expanding enzyme functionality through site-specific incorporation of noncanonical amino acids” Trends in Biochem Sci. 2025.
48. Kudalkar, G.P.; Leidner, F.; Kumar, N.; Hass, J.L.; Madzelan, P.; Powell, D.R.; Day, V.W.; LeMafueres, P.; Ferrara, J.D.; Daniels, L. M.; Yamano, A.; Ito, S.; Niu, W.; Grubmuller, H.; Wilson, M.A.; Berkowitz, D.B.”Is a malleable active site loop the key to high substrate promiscuity? Hybrid, biocatalytic route to structurally diverse taxoid side chains with remarkable dual stereocontrol” Angew. Chem. Int. Ed. 2025.
47. Niu, W.*; Guo J.* “Cellular site-specific incorporation of noncanonical amino acids in synthetic biology” Chem. Rev. 2024.
46. Agosto-Maldonado, A.; Guo, J., Niu, W.* “Engineering carboxylic acid reductases and unspecific peroxygenases for flavor and fragrance biosynthesis" J. Biotechnol. 2024.
45. Niu, W.*; Guo, J.* “Co-translational installation of posttranslational modifications by non-canonical amino acid mutagenesis” ChemBioChem 2023.
44. He, X.; Gao, T.; Chen, Y.; Liu, K.; Guo, J.*; Niu, W.* “Genetic code expansion in Pseudomonas putida KT2440” ACS Syn. Biol. 2022.
43. Mueller, J.; Willett, H.; Feist A.; Niu, W.* “Engineering Pseudomonas putida for syringyl aromatics utilization” Biotechnol. Bioeng. 2022.
42. Lim, H. G.; Rychel, K.; Sastry, A. V.; Mueller, J.; Niu, W.; Feist, A. M.; Palsson, B. O.* “Machine-learning from Pseudomonas putida transcriptomes reveals its transcriptional regulatory network” Metab. Eng. 2022, 72, 297-310.
41. Chen, Y.; He, X.; Ma, B.; Liu, K.; Gao, T.; Niu, W.*; Guo, J.* “Noncanonical amino acid mutagenesis in response to recoding signal-enhanced quadruplet codons” Nucleic Acids Res. 2022.
40. He, X.; Ma, B.; Chen, Y.; Guo, J*.; Niu, W.* “Genetic Encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells.” Chem. Commun. 2022, 58, 5897-5900.
39. Niu, W.; Guo, J.* “Genetic code expansion through quadruplet codon decoding” J. Mol. Biol. 2021.
38. Lawrie, J.; Waldrop S.; Morozov, A.; Niu, W.; Guo, J.* “Engineering of a small protein scaffold to recognize sulfotyrosine with high specificity.” ACS Chem. Biol. 2021, 16, 1508-1517.
37. He, X.; Guo, J.*; Niu, W.* “Studying protein tyrosine O-sulfation in mammalian cells with genetically encoded sulfotyrosine” Curr. Protoc. 2021.
36. He, X.; Chen, Y.; Beltran, D.G.; Kelly, M.; Ma, B.; Lawrie, J.; Wang, F.; Dodds, E.; Zhang, L.; Guo, J.*; Niu, W.* "Functional genetical encoding of sulfotyrosine in mammalian cells" Nature Communications 2020, 11:4820.
35. Kramer, L.; Le, X.; Rodriguez, M.; Wilson, M.A.; Guo, J.; Niu, W.* "Engineering carboxylic acid reductase (CAR) through a whole-cell growth-coupled NADPH recycling strategy" ACS Synthetic Biology 2020, 9, 1632-1637.
34. Niu, W.*; Willett, H.; Mueller, J.; He, X.; Ma, B.; Guo, J. “Direct biosynthesis of adipic acid from lignin-derived aromatics using engineered Pseudomonas putida KT2440” Metabolic Engineering 2020, 59, 151-161.
33. Nogales, J.; Mueller, J.; Gudmundsson, S.; Canalejo, F.J.; Duque, E.; Monk, J.; Feist, A.M.; Ramos, J.L.; Niu, W.; Palsson, B.O. “High-quality genome-scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities” Environmental Microbiology 2020, 22, 255-269. (¶ equal contribution)
32. Kramer, L.; Le, X.; Hankore, E.D.; Wilson, M.A.; Guo, J.; Niu, W.* "Engineering and characterization of hybrid carboxylic acid reductases" Journal of Biotechnology 2019, 304, 52-56.
31. Niu, W.*; Kramer, L.; Mueller, J.; Liu, K.; Guo, J. “Metabolic engineering of Escherichia coli for the de novo stereospecific biosynthesis of 1,2-propanediol through lactic acid” Metabolic Engineering Communications 2019, 8, doi.org/10.1016/j.mec.2018.e00082.
30. Hankore, E.; Zhang, L.; Chen, Y.; Niu, W.; Guo, J.* “Genetic incorporation of noncanonical amino acids using two mutually orthogonal quadruplet codons”, ACS Synthetic Biology, 2019, 8, 1168-1174.
29. Liu, Y.; He, X.; Lim, W.; Mueller, J.; Lawrie, J.; Kramer, J.; Guo, J.; Niu, W.* “Deciphering molecular details in the assembly of alpha-type carboxysome” Scientific Reports 2018, doi: 10.1038/s41598-018-33074-x.
28. Kramer, L.; Hankore, E. D.; Liu, Y.; Liu, K.; Jimenez, E.; Guo, J.*; Niu, W.* “Characterization of Carboxylic Acid Reductases for biocatalytic synthesis of industrial chemicals” ChemBioChem 2018, 19, 1452-1460.
27. Chen, Y.; Wan, Y.; Wang, N.; Yuan, Z.; Niu, W.*; Li, Q.*; Guo, J.* “Controlling the replication of a genomically recoded HIV-1 with a functional quadruplet codon in mammalian cells” ACS Synthetic Biology 2018, 7, 1612-1617.
26. Shang, X.; Chen, Y.; Wang, N.; Niu, W.; Guo, J.* “Oxidation-induced generation of a mild electrophile for proximity-enhanced protein–protein crosslinking” Chemical Communications 2018, 54, 4172 – 4175.
25. Lawrie, J.; Song, X.; Niu, W.*; Guo, J.* “A high throughput approach for the generation of orthogonally interacting protein pairs” Scientific Reports 2018, 8, doi.org/10.1038/s41598-018-19281-6.
24. Shang, X., Lai, R.; Song, X.; Li, H.; Niu, W.; Guo, J.* “Improved photoinduced fluorogenic alkene-tetrazole reaction for protein labeling” Bioconjugation Chemistry 2017, 28, 2859-2864.
23. Shang, X.; Wang, N.; Cerny, R.; Niu, W.; Guo, J.* “Fluorescent protein-based turn-on probe through a general protection-deprotection design strategy” ACS Sensors 2017, 2, 961-965.
22. Yuan, Z.; Wang, N.; Kang, G.; Niu, W.*; Li, Q.*; Guo, J.* “Controlling multi-cycle replication of live-attenuated HIV-1 using an unnatural genetic switch” ACS Synthetic Biology 2017, 6, 721-731.
21. Shang, X.; Song, X.; Faller, C.; Lai, R.; Li, H.; Cerny, R.; Niu, W.; Guo, J.* “Fluorogenic protein labeling using a genetically encoded unstrained alkene” Chemical Science 2017, 8, 1141-1145.
20. Wang, N.; Yuan, Z.; Niu, W.*; Li, Q.*; Guo, J.* “Synthetic biology approach for the development of conditionally replicating HIV-1 vaccine” Journal of Chemical Technology and Biotechnology 2017, 92, 455-462.
19. Ju, T.; Niu, W.*; Guo, J.* “Evolution of SH2 domain to recognize sulfotyrosine” ACS Chemical Biology 2016, 11, 2551-2557.
18. Song, X., Shang, X.; Ju, T.; Niu, W.*, Guo, J.* “A photoactivable Src homology 2 (SH2) domain” RCS Advances 2016, 6. 51120-51124.
17. Wang, N.; Shang, X.; Cerny, R.; Niu, W.*; Guo, J.* “Systematic evolution and study of UAGN decoding tRNA in a genomically recoded bacteria” Scientific Reports 2016, 6, doi.org/10.1038/srep21898.
16. Cao, J.; Cheng T.; Zhao, G.; Niu, W.; Guo, J.; Xian, M.; Liu, H.* “Metabolic engineering of Escherichia coli for the production of hydroxy fatty acids from glucose” BMC Biotechnology 2016, 16, doi: 10.1186/s12896-016-0257-x.
15. Niu, W.*; Guo, J. “Stereospecific microbial conversion of lactic acid into1,2-propanediol” ACS Synthetic Biology. 2015, 4, 378-382.
Prior to Department of Chemical and Biomolecular Engineering at UNL
14. Tong, W.; Xu, Y.; Xian, M.; Niu, W.; Guo, J.; Liu, H.; Zhao, G.* “Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli” Applied Microbiology Biotechnology 2016, 100, 4901-4907.
13. Cao, Y.; Niu, W.; Guo, J.; Xian, M.; Liu, H.* “Biotechnological production of 1,2,4-butanetriol: an efficient process to synthesize energetic material precursors from renewable biomass” Scientific Reports 2015, 5, doi.org/10.1038/srep18149.
12. Wang, N.; Ju, T.; Niu, W, Guo, J.* “Fine-tuning Interaction between Aminoacyl-tRNA Synthetase and tRNA for Efficient Synthesis of Protein Containing Unnatural Amino Acids” ACS Synthetic Biology 2015, 4, 207-212.
11. Wang, N.; Li, Y.; Niu, W.; Sun, M.; Cerny, R.; Li, Q.; Guo, J.* “Construction of a live-attenuated HIV-1 vaccine through genetic code expansion” Angewandte Chemie International Edition 2014, 53, 4867-4871.
10. Niu, W.; Guo, J.* “Expanding the chemistry of fluorescent protein biosensors by genetic incorporation of unnatural amino acids” Molecular BioSystems 2013, 9, 2961-2970.
9. Niu, W.; Schultz, P. G.; Guo, J.* “An expanded genetic code in mammalian cells with a functional quadruplet codon” ACS Chemical Biology 2013, 8, 1640-1645.
8. Ju, T.; Niu, W.; Ronald Cerny; Bollman, J.; Roy, A. Guo, J.* “Molecular recognition of sulfotyrosine and phosphotyrosine” Molecular Biosystems 2013, 9, 1829 - 1832.
7. Wang, G.; Xu, Z.; Chen, Z.; Niu, W.; Zhou, Y.; Guo, J.; Tan, L. “Sequential binding of large molecules to hairy MOFs” Chemical Communications 2013, 49, 6641-6643.
6. Wang, F.; Niu, W.; Guo, J.*; Schultz, P. G.* “Unnatural amino acid mutagenesis of fluorescent proteins” Angewandte Chemie International Edition 2012, 51, 10132-10135. https://doi.org/10.1002/anie.201204668
5. Park, C. M.; Niu, W.; Liu, C.; Biggs, T. D.; Guo, J.; Xian, M. “A proline-based phosphine template for Staudinger ligation” Organic Letters 2012, 14, 4694-4697.
Prior to University of Nebraska-Lincoln
4. Yim, H.*; Haselbeck, R.*; Niu, W.*; Pujol-Baxley, C.*; Burgard, A.*; Boldt, J.; Khandurina, J.; Trawick, J. D.; Osterhout, R. E.; Stephen, R.; Estadilla, J.; Teisan, S.; Schreyer, H. B.; Andrae, S.; Yang, T. H.; Lee, S. Y.; Burk, M. J.; Van Dien, S. “Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol” Nature Chemical Biology 2011, 7, 445-452. * These authors contributed equally to this work. (This article was highlighted in this issue of Nature Chemical Biology)
3. Niu, W.; Molefe, M. N.; Frost, J. W.* “Microbial synthesis of the energetic material precursor 1,2,4-butanetriol” Journal of the American Chemical Society 2003, 125, 12998-12999.
2. Niu, W.; Draths, K. M.; Frost, J. W.* “Benzene-free synthesis of adipic acid” Biotechnology Progress 2002, 18, 201-211.
1. Niu, W.; Wu, Yi; Sui, Sen-Fang “Orientation of membrane-bound melittin studied by a combination of HPLC and liquid secondary ion mass spectrometry” IUBMB Life 2000, 50, 215-219.